Abstract: Infrared spectroscopy is one of the methods used in the Chinese Pharmacopoeia to identify the structure of compounds.Mastering the experimental skills of infrared spectroscopy and the ability to analyze infrared spectroscopy are the basic requirements for undergraduates majoring in pharmacy.Experimental teaching is an important practical teaching link in pharmacy education,and it plays an irreplaceable role in cultivating students' practical ability,knowledge application ability and innovation ability.The author breaks the traditional infrared experiment teaching method,uses micro-video technology to explore and establish an online teaching mode integrating basic experiment,instrument operation and spectrum analysis,which is no longer limited by offline operation,so that students can better understand the principle of infrared experiments,master the operation of the instrument more proficiently,improve the ability to accurately analyze the spectrum,and then comprehensively improve the comprehensive learning ability of students.