Abstract: Traditional Chinese medicine culture is a historical and cultural treasure of China.Medicinal botanical gardens,as effective vehicles for show casing and expressing Traditional Chinese medicine culture,are increasingly recognized for their importance.Using the Medicinal Botanical Garden of China Pharmaceutical University as a case study,this paper discusses the approaches and methods for constructing Traditional Chinese medicine culture in university-affiliated professional medicinal botanical gardens.It also explores the value,significance,and impact of these methods in Traditional Chinese medicine education and public science education,providing a basis and reference for the construction of Traditional Chinese medicine culture in university-affiliated medicinal botanical gardens.