Abstract: Pharmaceutical analysis is an important professional course for pharmacy, and a highly practiceoriented subject. Experimental teaching is an integral part of teaching, while traditional experimental teaching can hardly meet the needs of modern teaching. The teaching team explored the application of virtual simulation combined with PBL teaching method to experimental teaching, constructing a relatively established curriculum evaluation system from experimental teaching content, teaching methods and experimental assessment, which reformed and pioneered the experimental teaching of pharmaceutical analysis courses, so as to cultivate innovative and compound talents versed in pharmacy.