Abstract: As a newly emerging subject integrated by multidiscipline,biopharmaceutical analysis is one of the specialized courses of pharmacy undergraduates. In this paper,the authors discuss the exploration and experience on theoretical and experimental teaching of biopharmaceutical analysis in recent years from the aspects of teaching material and content selection,teaching-learning interactions,curricular ideological and political education,experiment design and arrangement,course evaluation and teaching effectiveness assessment. The purpose of this research is to enhance teaching effectiveness of the course and improve the cultivation quality of undergraduates.