Abstract: Chemical biology is an interdisciplinary subject that explores life phenomena through chemical means, which role in the field of medicine is becoming increasingly prominent.It is a required course for innovative pharmaceutical talents.In order to allow students to better grasp the knowledge of chemical biology in an effective time, learn to apply chemical biology techniques and theories to solve problems encountered in the process of innovative drug discovery, a instrument-oriented chemistry was carried out for students majoring in medicinal chemistry.Combined with the results of teaching practice, it is found that the teaching of chemical biology courses highlighting the instrumental characteristics of the discipline is conducive to cultivating students' ability to apply chemical biology thinking to solve problems.