Abstract: In order to improve the teaching effect of online courses, an exploration of online teaching reform of instrumental analysis course was carried out based on Chaoxing SPOC platform+Learning Pass+Tencent Conference+Instant Social Software, and the "pre-course introduction-in-class lecture-post-course practice-online learning" model was constructed.This model breaks the time and space barriers between teaching and learning, stimulates teachers' teaching enthusiasm, drives students' learning motivation, strengthens teachers' and students' interaction and communication, enhances the classroom vitality and improves the teaching effect of the course.