Abstract: The transformation of drug research and development from imitation to original research in China has put forward new requirements for the reform of the teaching mode of pharmacy-related experimental courses.In order to improve the teaching effect of the pharmacokinetics experimental course, the Journal of Visualized Experiments (JoVE) science and education video library was combined with the existing comprehensive design experimental course system to optimize the experimental teaching resources and expand the second classroom learning space.The results show that the JoVE science and education video library can help stimulate students' interest in learning, improve the success rate of experiments, increase students' understanding of experimental principles, and improve their ability to solve practical problems.