Abstract: Pharmaceutical chromatographic analysis experiment is an important basic experimental course for pharmacy students.The previous experimental teaching mode lacked effective teaching methods, and it was difficult to meet the needs of enterprises for pharmacy talents with excellent scientific literacy.This paper analyzes the problems existing in the teaching mode of traditional pharmaceutical chromatography analysis course, discusses the necessity of reforming the teaching mode, and constructs a new experimental teaching mode based on APP teaching platform, virtual simulation experiment teaching platform and open experimental teaching platform, so as to cultivate students' innovative consciousness and improve students' practical ability.