Abstract: Taking the construction of excellent courses in Jiangsu Province as an opportunity, on the basis of referring to the experience of domestic medical students' biochemistry teaching reform, combined with the actual situation, the reform was carried out in many aspects, such as teacher selection, teaching model reform, teaching resource construction, and compound assessment method establishment to establish a new biochemistry teaching mode combining online and offline with the characteristics of Nanjing Medical University, in order to improve the effect of biochemistry teaching, improve the quality of the school's international students training, and the school's international influence and the implementation of China's Belt and Road Initiative.