Abstract: Taking the "Pharmaceutical Quality Analysis and Control" course from the Pharmacy (Integrated Undergraduate-to-Doctoral Elite Experimental Class) at China Pharmaceutical University as an example,this paper introduces the construction and practice of a curriculum system aimed at cultivating top-tier innovative pharmacy talents under the framework of the "Top-notch Talent Program" 2.0. With the core goal of fostering professionals who are "proficient in medicine and pharmacy,skilled in research and application",the course addresses key teaching challenges such as the extensive content,abstract and complex fundamental principles,and students' limited ability to solve practical problems in pharmaceutical analysis.The teaching philosophy of "learning for application,applying to enhance learning,and integrating learning and application" is proposed.The course integrates and streamlines related topics from instrumental analysis and pharmaceutical chromatographic analysis,creating a specialized exploratory course in pharmaceutical quality analysis and control.Additionally,a series of teaching reform practices incorporating digital resources have been implemented to provide valuable insights for cultivating top-notch innovative talents in pharmacy.