Abstract: With the rapid development of the social economy and the continuous upgrading of industrial structures,the demand for high-quality skilled talents is increasingly urgent.The "Five Golden" (golden specialties,golden teachers,golden courses,golden sites,and golden textbooks) reform has emerged in response to this demand.Medicinal Chemistry,as the foundational discipline for drug development,must update course content,innovate teaching methods,and improve the evaluation system under the "Five Golden" framework.This paper,based on the teaching practices of higher vocational colleges and combined with the characteristics of Medicinal Chemistry courses,explores innovations in theoretical teaching,experimental teaching,assessment and evaluation to provide a reference for frontline teaching of similar courses.