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高等教育研究学术期刊
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天然药物化学实验中液质联用技术的系统化教学设计与实践

Systematic Teaching Design and Practice of Liquid Chromatography-Mass Spectrometry Technology in Natural Product Chemistry Experiments

  • 摘要: 基于原有的天然药物化学教学实验,本研究引入液质联用技术,分别建立了以下方法:利用单四极杆质谱的-峰鉴定芦丁,采用单标法测定其纯度;利用单四极杆质谱的M++2+峰鉴定小檗碱和小檗胺,采用外标法测定其纯度;利用四极杆串联飞行时间质谱鉴别大黄酚、大黄酸和大黄素甲醚,通过二级质谱碎片离子的差异鉴别同分异构体大黄素和芦荟大黄素。3个实验的教学设计以问题为导向,覆盖了液质联用技术的基本知识点,形成由浅入深、各有侧重的学习体系。新实验教学设计方案易与原有实验接轨,实现了液质联用技术在本科教学中的落地应用,提高了学生的实验技能。

     

    Abstract: Building upon the existing experimental curriculum in natural product chemistry,this study introduces liquid chromatography-mass spectrometry (LC-MS) techniques through the development of following experimental modules:single quadrupole mass spectrometry was used to identify rutin via its M-H- peak.Its purity was determined using a single-standard method.The M+,M+H+,and M+2H2+ peaks were used to identify berberine and berberine amine.Their purity was measured through an external standard method.Chrysophanol,rhein,and physcion is identified by quadrupole time-of-flight tandem mass spectrometry.Isomeric compounds,such as aloe-emodin and emodin,were distinguished based on fragmentation ion patterns in their secondary mass spectra.The instructional design of these experiments is problem-driven and systematically covers key LC-MS knowledge points,forming a progressively structured learning framework with distinct emphases.The new experimental modules integrate seamlessly with the original curriculum,effectively embedding LC-MS technology into undergraduate education and enhancing students' experimental competencies.

     

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